Used BIO-RAD CFX96 Connect

CFX Connect Real-Time PCR Detection System

The CFX Connect Real-Time PCR System provides excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.

Specifications:

Chassis: CFX Connect
Maximum ramp rate, °C/sec: 5
Average ramp rate, °C/sec: 3.3
Heating and cooling method: Peltier
Lid, °C: Heats up to 105
Temperature:

Range, °C: 0-100
Accuracy, °C: ±0.2 of programmed target at 90°C
Uniformity, °C: ±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient:

Operational range, °C: 30-100
Programmable span, °C: 1-24
Optical Detection:

Excitation: 3 filtered LEDs
Detection: 3 filtered photodiodes
Range of excitation/emission wavelengths, nm: 450-580
Sensitivity: Detects 1 copy of target sequence in human genomic DNA
Dynamic range: 10 orders of magnitude
Scan Time:

All channels, sec: 12
FAM/SYBR® Green only, sec: 3
Software:

Operating systems: Windows 7, Windows 8, Windows 10
Multiplex analysis: Up to 2 targets per well
System:

Licensed for real-time PCR: Yes
Sample capacity, wells: 96
Sample size, µl: 1–50 (10–25 recommended)
Communication interface: USB 2.0
Electrical approvals: IEC, CE
Dimensions (W x L x H), cm/in: 33 x 46 x 36/13 x 18 x 14
Weight, kg/lb: 21/47