Used MOLECULAR DEVICES SpectraMax iD3

The SpectraMax iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF, BRET, dual luciferase reporter assays with injectors, and western blot detection. With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax Pro Software, the readers provide an all access pass for breakthrough research.

Specification:

Read modes:
Absorbance
Fluorescence (top/bottom read),
Luminescence (top read)
Wavelength ranges:
Abs: 230 – 1000 nm
FL Ex: 250 – 830 nm
FL Em: 270 – 850 nm
Lumi: 300 – 850 nm
Wavelength selection: Monochromator tunable in 1-nm increments
Absorbance photometric accuracy/linearity: < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Absorbance photometric precision/repeatability: < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 3 OD UV
Sensitivity top read optimized:
FL:1 pm fluorescence 96 well
1 pm fluorescein 384 well
Lumi: 2 pm ATP 96 well, 4 pm
Light source(s): High power xenon flash lamp
Detector(s): Silicon photodiode, Ultra-cooled photomultiplier tube
Read types (endpoint, kinetic, etc): Endpoint, Kinetic, Spectrum scan, Well scan
Read times:
Abs: 28 seconds,
FL: 21 seconds
Lumi: 21 seconds
Plate shaking: Linear, Orbital, Double orbital
Plate type(s): 6-384 well plates
Temperature control: Ambient + 5°C to 66°C
Dimensions: H 40.1 cm x W 53.2 cm x D 59.8 cm
Validation tools:
Absorbance validation plate
Fluorescence validation plate
Luminescence validation plate
SoftMax Pro GxP Software