Agilent BioTek Synergy H1 Microplate Reader H1MFG

Internal part number 63506

For Sale Agilent BioTek Synergy H1 Microplate Reader H1MFG

• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
• 60 DAYS INTERNATIONAL WARRANTY

Here is what you will receive:

• BioTek Synergy H1M Microplate Reader
• Communication and power cables

Please note that the computer with the Gen5 software used for testing is not included and will need to be purchased separately.

BioTek Synergy H1 Microplate Reader Configuration:

• Wavelength Selection: Quadruple grating monochromator system and Filter cube sets (Hybrid system) (Filters are not included)
• Detection Modes: UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence
• Read method: Endpoint, kinetic, spectral scanning, well area scanning
• Microplate types: 6-, 12-, 24-, 48-, 96-, and 384-well
• Temperature control: up to 45 DegC; /-0.2 DegC at 37 DegC
• Shaking: Linear, orbital, double orbital

Absorbance

• Light source: Xenon flash lamp
• Wavelength selection: Monochromator
• Wavelength range: 230 - 999 nm, 1 nm increment
• Bandpass: 4 nm (230 - 285 nm), 8 nm (>285 nm)
• Dynamic range: 0 - 4.0 OD
• Resolution: 0.0001 OD
• Pathlength correction: Yes
• OD accuracy: <1 % at 2.0 OD, <3% at 3.0 OD
• OD repeatability: <0.5 % at 2.0 OD
• Reading speed: 96 wells: 11 seconds, 384 wells: 22 seconds

Fluorescence Intensity

• Light source: Xenon flash lamp
• Detection system: PMT detectors
• Wavelength selection: Double grating monochromators (Top and Bottom) and Filters (top)
• Wavelength range: Monochromators: 250 - 700 nm; Filters: 200 – 700 nm
• Dynamic range: 5 decades
• Sensitivity Monochromators: Top: Fluorescein 2.5 pM (0.25 fmol/well 384-well plate) Bottom: Fluorescein 4 pM (0.4 fmol/well 384-well plate)
• Sensitivity Filter system: Fluorescein 0.25 pM (0.025 fmol/well 384-well plate)

Luminescence

• Sensitivity: Monochromator system 20 amol ATP (flash), Filter system: 10 amol ATP (flash)
• Wavelength range: 300 - 700 nm
• Dynamic range: >6 decades

Fluorescence Polarization (Requires the purchase of FP filter set)

• Light Source: Xenon flash 
• Detector: PMT
• Wavelength Selection: Filters
• Sensitivity: 1.2 mP standard deviation at 1 nM fluorescein
• Wavelength range: 320 - 700 nm

Time-Resolved Fluorescence

• Light source: Xenon flash lamp
• Europium 1200 fM with monos (120 amol/well in 384-well plate)
• Wavelength range: Monochromators: 250 - 850 nm; Filters: 200 - 700 nm

Customization/Options:

• BioTek BioStack Microplate Stacker for higher throughput

Testing performed by our in-house service team:

The BioTek Synergy H1 Microplate Reader passed all tests and was found to be fully functional:

• We verified the power-on functionality.
• We confirmed successful system initialization.
• We tested the connection to the control software.
• We assessed the movement of the plate carrier.
• We tested the incubator performance up to the maximum temperature.
• We verified the operation of the shaking function.
• We tested fluorescence using a fluorescence test plate.
• We tested luminescence using a luminescence test plate.
• We tested absorbance using an absorbance test plate.

Instrument Overview:

• BioTek Synergy H1 Microplate Reader H1MFG combines monochromator and filter-based detection optics.
• Supports fluorescence, luminescence, and UV-Vis absorbance measurements with high sensitivity.
• Variable bandwidth selection enhances spectral flexibility and precision across assays.
• Automated Z-focus optimizes read height for different plate types and sample volumes.
• Provides extended dynamic range for accurate detection of weak and strong signals.
• Compact modular design allows future upgrades for injectors and gas control options.

Instrument Application:

• Quantification of fluorescence and luminescence assays
• Absorbance-based enzyme and kinetic measurements
• Nucleic acid and protein concentration determination
• Cell-based assay and reporter gene analysis

The Key Features:

• Combines monochromator and filter-optics hybrid detection
• Variable bandwidth between 9–50 nm in 1 nm steps
• Automated Z-focus adjusts read height for different plates
• Extended dynamic range covers weak to strong signals
• Top and bottom read capability for fluorescence
• Modular, upgradeable for injectors or gas control

Our Advantage

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.  see the questions section below  
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Technical sales consultation available
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

SM Small Crate
200#

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper.  If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.  

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries. 

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.