Zeiss AXIO Observer.A1 Inverted Research Microscope, Phase Contrast & PlasDIC Configuration

Internal part number 64758

For Sale Zeiss AXIO Observer.A1 Inverted Research Microscope, Phase Contrast & PlasDIC Configuration

• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters 
• Technical sales consultation available
• 60 DAYS INTERNATIONAL WARRANTY
• Includes remote microscope training session and software overview

Service Highlights

• Optics quality is checked for field distortion and uniformity at the specimen plane
• Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide at our Website

This is what you will receive before customizing this microscope:

Standard Zeiss AXIO Observer.A1 Inverted Microscope Configuration: 

Microscopy Techniques:

Brightfield and Phase Contrast Microscopy, PlasDIC

Microscope Base:

• Zeiss AXIO Observer.A1 Inverted Microscope

Microscope Head:

• Binocular tube
• W-PI 10x/23 Eyepieces (Qty. 2)

Brightfield Light Path:

• Brightfield 100W Halogen Lamp

Objective Turret:

• Six-Channel Manual Objective Turret

Objectives:

• Zeiss 5x/0.16 Ph1 EC Plan-NEOFLUAR
• Zeiss 10x/0.3 Ph1 EC Plan-NEOFLUAR
• Zeiss 20x/0.5 Ph2 EC Plan-NEOFLUAR
• Zeiss 40x/0.75 Ph2 EC Plan-NEOFLUAR w/ PlasDIC.

Condenser:

• Mechanical 6-Position Condenser w/ Phase Contrast and PlasDIC
• NA 0.55, WD 26mm Condenser Lens

Stage:

• Mechanical XY Stage

Camera ports:

• Eyepieces - 100%
• Left port - 100%

Camera:

• BestScope 8.3MP color COS Camera (model: BUC5F-830EC)

Computer & Software:

• Computer running applicable software

Accessories:

• Necessary power and communication cables

PlasDIC Configuration:

• This system is currently set up for 40x PlasDIC imaging, a variation of DIC designed specifically for plastic vessels.
• Utilizes a slotted condenser insert instead of a traditional DIC prism.
• Requires a PlasDIC-compatible objective prism (included at 40x).
• Optimized for imaging live cells in plastic cultureware.
• The slotted condenser insert is specialized and typically not used outside of PlasDIC applications.

This microscope's imaging capabilities:

Imaging software highlights:

• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others 
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time) 

We can customize this microscope to fit your requirements

Add Microscopy Techniques (In addition to installed Brightfield and Phase Contrast)

• Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality: 

• Microscope can be configured for magnification between 50x and 400x (with 10x Eyepieces)
• We can add standard higher magnification Zeiss microscope objectives 
• We can add high-end Zeiss Apochromat objectives

Enhance imaging capabilities: 

• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

Add other Motorized/Automation Capabilities: 

In addition to installed motorized capabilities, the microscope can be configured

• Motorized Z-axis (focus drive)
• Motorized objective turret
• Motorized condenser
• Motorized XY stage

We can configure it to work with the sample medium you need:

• Slide, Petri dish, microplate, etc.
• It may also be set up with a motorized XY stage for multi-slide or full microplate scans.

Commonly Requested Customer Upgrades

• High-End CCD, sCMOS Cameras
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Zeiss AXIO Observer.A1 Inverted Microscope Overview 

(Final microscope capabilities will depend on the modifications you request)

• The Zeiss AXIO Observer.A1 is an inverted microscope configured for brightfield, phase contrast, and 40x PlasDIC imaging.
• PlasDIC is optimized for live-cell imaging in plastic culture dishes, using a slotted condenser insert instead of a traditional DIC prism.
• This system includes EC Plan-NEOFLUAR objectives from 5x to 40x, with the 40x configured for PlasDIC.
• A 6-position condenser supports both phase contrast and PlasDIC applications.
• Illumination is provided by a 100W halogen lamp with a tiltable illumination arm.
• An 8.3MP color CMOS camera and computer are included for digital imaging and documentation.
• Precision mechanical XY stage for smooth and stable sample positioning.

Suited for the following applications as configured:

• Routine brightfield cell culture observation
• Phase contrast imaging of live, unstained cells
• Basic fluorescence imaging (with appropriate filter sets)
• Cell morphology and confluency assessment
• Educational and research laboratory microscopy
• General life science and biomedical imaging workflows

Other potential applications:

• Time-lapse imaging with environmental control upgrades
• Multi-channel fluorescence studies with additional filter sets
• 3D Z-stack imaging with motorized focus integration
• Multi-position imaging with motorized XY stage
• DIC imaging with compatible optics
• Integration with confocal or advanced imaging modules

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

• They want to ensure the microscope is customized to fit their application 
• They want to ensure that internal optics are properly cleaned 
• They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
• They want to ensure that image quality is verified at the sample point for field distortion and uniformity
• They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
• They want an option to upgrade the camera to sCMOS.
• They want training to learn how to operate the microscope 
• They want a reliable warranty
• They want peace of mind knowing it will be safely and professionally packed for shipping

Our Advantage

• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

SM Small Crate
300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application 

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives. 

Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past? 
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.