Used ZEISS Axio Observer 3

Zeiss Axio Observer 3 Inverted Phase Contrast Fluorescence Binocular Microscope (Model 431004-9901-000)

The Zeiss Axio Observer 3 is a manually operated inverted fluorescence microscope built for core research, cell culture imaging, and fluorescence applications. Designed with Zeiss acclaimed infinity-corrected optics and configured for phase contrast, this system delivers excellent image clarity and reliable performance for live-cell and fixed sample imaging.

This configuration includes a binocular viewing head (standard for Observer 3), phase contrast condenser, manual optical components, and a manual stage with an optional motorized XY upgrade for enhanced control. A manual condenser is included, offering straightforward contrast control for transmitted light and phase contrast work. This system is ideal for labs that need robust imaging capabilities without full automation.

Condition

Refurbished/Used (This product has been inspected and verified to meet manufacturer specifications.)

Included Items

• Zeiss Axio Observer 3 Microscope Body (Model 431004-9901-000, Inverted)
• Binocular Viewing Head – Standard ergonomic design for extended visual work
• Widefield Eyepieces – High eye-point for clear, comfortable viewing
• Manual Z-Focus and Manual Nosepiece – Smooth focus and objective switching
• Manual Fluorescence Filter Cube Turret – Includes 3 filter cubes of your choice
• Manual Condenser – Standard Abbe-style for transmitted light and phase applications
• Phase Contrast Condenser and Optics – For high-contrast imaging of live, unstained cells
• Transmitted Light Arm and Power Supply
• Manual XY Stage – Optional motorized upgrade available
• Left-Side C-Mount Camera Port – Compatible with most scientific cameras

Objectives

(Please discuss your preferred configuration with our team. We offer a full suite of Zeiss objectives including Plan-Apochromat, EC Plan-Neofluar, and Phase options.)

Fluorescence Illumination Options

Illuminator (Choose One):

• 100w Mercury with Power Supply
• Upgrade to: Used Metal Halide
• Upgrade to: New X-Cite 110LED System (Includes Adapter, speedDIAL Remote, 3mm x 1500mm Liquid Light Guide)
• Upgrade to: New X-Cite mini+ System (Includes LED Head, miniCUBE Controller, speedDIAL Remote, Microscope Coupling Flange)
• Upgrade to: New X-Cite XYLIS II XT730S (Includes XT720S, speedDIAL Remote, 3mm x 1500mm Liquid Light Guide)

(3) Filter Cubes of Your Choice

(Additional cubes available for purchase.)

Key Features

• Manual Inverted Microscope Platform – Durable and dependable for routine and research labs
• Zeiss Infinity-Corrected Optical System – Delivers sharp, high-contrast images
• Manual Condenser – Simple and effective light control for transmitted and phase illumination
• Phase Contrast Imaging – Enhances visualization of live, unstained cells
• Fluorescence Capable – For imaging with DAPI, FITC, TRITC, and more
• Binocular Viewing Head – Standard for all Observer 3 units
• Camera Integration – Compatible via standard side C-mount port

Applications

• Live Cell Imaging
• Phase Contrast Observation
• Fluorescence Microscopy
• Cell Culture Monitoring
• Fixed Sample Analysis
• Biomedical Research and Teaching Labs

Specifications

Feature	Specification
Microscope Type	Inverted, Manual Operation
Catalog Number	431004-9901-000
Viewing Head	Binocular
Focus	Manual Z-Focus
Nosepiece	Manual, typically 5-position
Stage	Manual XY (Motorized Upgrade Available)
Filter Cube Turret	Manual, 3 positions included
Condenser	Manual
Phase Contrast	Yes
Illumination Options	Mercury, Metal Halide, or LED
Optical System	Zeiss Infinity-Corrected
Camera Port	Left-Side C-Mount
Camera Compatibility	Yes, Optional
Software	Not included
Computer	Not included

Configuration shown is for demonstration purposes. Contact us to discuss your application and needs for a customized quote.