For Sale Nikon Eclipse Ni-U Upright Fluorescence Phase Contrast Microscope with Motorized Stage

Internal part number SM100185-XY

• Fully Functional
• Serviced & in Stock
• Customized to your requirements
• Includes new fluorescence filters 
• Technical sales consultation available
• 60 DAYS INTERNATIONAL WARRANTY
•  Optional upgrade to 1 YEAR WARRANTY for $1995.00
•  Includes remote microscope training session and software overview

Service Highlights

• Optics quality is checked for field distortion and uniformity at the specimen plane
• Fluorescence Intensity is also measured at the specimen plane to confirm light source and filter performance
• Preventive Maintenance (PM) is Completed

More info about our Microscopy Capabilities or view the Microscope Buying Guide at our Website

This is what you will receive before customizing this microscope:

Standard Nikon Eclipse Ni-U Upright Microscope Configuration: 

Microscopy Techniques:

Brightfield with Phase Contrast and Fluorescence Microscopy

 Microscope Base:

• Nikon Eclipse Ni-U Upright Microscope

Microscope Head:

• Trinocular head (C-TT)
• 2 HC Plan s 10x/22 Optics
• Camera Mount 

Fluorescence Light Path:

• Base

Fluorescence Illumination:

• X-Cite 120PC Q Metal Halide

Fluorescence filter cube turret:

• Manual Ni-FLT6 6-position turret
• Light shielding plate

Fluorescence filters:

• Three empty filter cubes
• Three Brand New filter sets of either DAPI, FITC, Texas Red, or CY3 of either DAPI, FITC, Texas Red, or CY3

Brightfield Light Path:

• Base

Brightfield Illumination:

• Halogen 12V100W (NI-LH) Lamp

Objective Turret:

• 6-Position Manual Objective Turret (C-N6 ESD)

Objectives:

• Nikon 4x Plan Fluar PH
• Nikon 10x Plan Fluar PH
• Nikon 20x Plan Fluar PH

Condenser:

• Manual 7-Channel Condenser (NI-CUD)
• Phase Contrast Inserts (Ph1, Ph2, & Ph3)

Stage:

• Motorized XY Stage w/ mechanical Z-axis
• MCX-2 eco controller with joystick

Camera ports:

• 100% Eyepiece, 0% Camera
• 50% Eyepiece, 50% Camera
• 0% Eyepiece, 100% Camera

Camera:

• BestScope 8.3MP color CMOS Camera (BUC5F-830EC)

Computer & Software:

• Computer running applicable camera software

Accessories:

• Necessary power and communication cables 

This configuration includes any 3 of these new filter sets. 

• New filter set - DAPI (EX: 365/28nm, DM: 405nm, EM: 445/50nm) (Dye compatibility: DAPI/Hoechst/AlexaFluor 350)
• New filter set - EGFP/FITC/Cy2 (EX: 475/30nm, DM: 500nm, EM: 530/40nm) (Dye compatibility: EGFP/FITC/Cy2/AlexaFluor 488)
• New filter set - TRITC/Cy3/Dsred (EX: 545/20nm, DM: 562nm, EM: 605/60nm) (Dye compatibility: TRITC/Cy3/RFP/Rhodamine/AlexaFluor 546)
• New filter set - Texas Red (EX: 560/20nm, DM: 585nm, EM: 630/40nm) (Dye compatibility: Texas Red/mCherry/AlexaFluor 594)
• New filter set - Cy5 (May require an upgraded light source) (EX: 630/50nm, DM: 662nm, EM: 695/50nm) (Dye compatibility: Cy5/AlexaFluor 647/Draq5)

This microscope's imaging capabilities: 

Imaging software highlights:

• Ability to take still images and live videos of your samples
• Display, annotate, edit, calibrate, measure, analyze, process, print, and save raster
• Variable linear measurements such as line segment, radius values, and others 
• Open source Micromanager Multi-dimensional Image acquisition & automation software capabilities
• Multi-frame (Time lapse/Image acquisition over time) 
• Multi-slice (Z coordinate/Z Stacking) 

We can customize this microscope to fit your requirements

Add Microscopy Techniques (In addition to installed Brightfield, Phase Contrast and Fluorescence)

• DIC, or Polarization Add-on Components may be added (subject to availability)

Increase Maximum Magnification and Image Quality: 

• Microscope can be configured for magnification between 40x and 1000x (with 10x Eyepieces)
• We can add standard higher magnification Nikon microscope objectives 
• We can add high-end Nikon Apochromat objectives

Enhance fluorescence capabilities by adding/changing filters and upgrading light sources.

• Additional filter cubes 
• Additional filter sets to match your fluorochromes.
• Xenon, Metal Halide, or LED Fluorescence microscope light sources

We will also ensure the fluorescence light source matches your selected filter sets.

Enhance imaging capabilities: 

• We offer various advanced CCD, sCMOS, and EMCCD camera options for higher-quality images.

We can configure it to work with the sample medium you need:

• Slide, Petri dish, microplate, etc.

Commonly Requested Customer Upgrades

• High-End CCD, sCMOS Cameras
• LED or Metal Halide Fluorescence Light Sources
• Phase Contrast, Polarization, or the DIC configurations
• Higher Magnification Objectives
• Higher performance Fluor or APO objectives
• Motorized XY Stage (stage, controller, joystick)

We are excited to help build and configure the right microscope for you!

Nikon Eclipse Ni-U Upright Microscope Overview 

(Final microscope capabilities will depend on the modifications you request)

• Upright research microscope supporting brightfield, phase contrast, and fluorescence imaging.
• Trinocular viewing head with widefield optics and flexible eyepiece and camera port configurations.
• Fluorescence illumination with external light source and multi-position filter cube turret.
• Transmitted light illumination with phase contrast condenser and multiple phase ring inserts.
• Multi-position objective turret with phase-compatible objectives for routine microscopy.
• Motorized XY stage with manual Z-axis control for precise and repeatable sample positioning.

Suited for the following applications as configured:

• Routine brightfield and phase contrast imaging of unstained samples
• Fluorescence imaging using common dye sets
• Cell and tissue morphology observation
• Manual and motorized slide scanning workflows
• General research, teaching, and documentation use

Other potential applications:

• Time-lapse imaging with additional environmental accessories
• Multi-channel fluorescence with expanded filter sets
• Automated imaging with advanced stage or focus upgrades
• Quantitative image analysis with higher-sensitivity cameras
• Specialized contrast techniques with additional optical modules

REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US

• They want to ensure the microscope is customized to fit their application 
• They want to ensure that internal optics are properly cleaned 
• They want to ensure that internal mechanical and motorized mechanisms are lubricated and aligned
• They want to ensure that image quality is verified at the sample point for field distortion and uniformity
• They want to ensure that the fluorescence intensity is verified at the sample point 
• They want to ensure NEW fluorescence filters are installed just the same as buying a new microscope
• They want to ensure that if paired with a motorized XY stage, it is lubricated and does not skip steps
• They want an option to upgrade the camera to sCMOS and the light source to Lumencor or X-Cite LED
• They want training to learn how to operate the microscope 
• They want a reliable warranty
• They want peace of mind knowing it will be safely and professionally packed for shipping

Our Advantage

• We make sure to match the microscope capabilities to your requirements.
• We have experience servicing and repairing these microscopes.
• This microscope goes through an extensive set of service steps before shipping.
• We offer detailed photos and videos of the microscopes you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Many upgrade and repair parts are in stock.
• Remote installation and microscope operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• Expert US or International shipping is available (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Customer references are available.
• The microscope will be complete and working!
• Smooth process from the initial quote to order fulfillment.

SM Small Crate
300#

PLEASE NOTE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: I am not 100% sure about the configuration I need, can your team assist me with selecting the right microscope for me?
A: Absolutely, we can start with your application, samples, or challenge to assist you in selecting the right configuration and the right microscope for you.

Q: Can you make the necessary modifications to the standard configuration to fit my application?
A: Certainly, we carry a large variety of upgrade components to ensure the final microscope configuration will fit your application 

Q: What steps do you take to verify, service, and confirm the functionality of this Microscope?
A: Each microscope goes through extensive service to ensure it performs to specification.

Q: Can I view this microscope in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The microscope comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to get you familiar with this microscope

Q: What should I watch out for when buying a used/pre-owned microscope from another lab?
A: That it has no missing, broken, or incorrect parts for your application, any mechanical or motorized components have been serviced and lubricated, controller issues, good quality filter cubes, proper and undamaged objectives, and most importantly no dust in the light path or objectives. 

Q: Do you have these and other microscopes on hand?
A: We carry this and many other microscopes from Nikon, Zeiss, Olympus, and Leica.

Q: I am from the University or College, do you accept Purchase Orders?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within 2-3 days to about 2 weeks, depending on the final configuration required.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.

Q: Have you packed and shipped microscopes in the past? 
A: We have extensive experience in safely shipping high-end and complex microscopes all over the world.

Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new one, you will save up to 60% to 80% and have plenty of funds left over for an additional instrument or two.