For Sale Agilent 5300 Fragment Analyzer

Internal part number SM100786-5300B

• Fully Functional
• Serviced & in Stock
• Current Model
• Good cosmetic condition
• Technical sales consultation available
• 60 DAYS INTERNATIONAL WARRANTY
• Includes remote instrument setup and training session 

Here is what you will receive:

• Agilent 5300 Fragment Analyzer
• (4) Marker/sample PCR microplate (semi or non-skirted) holders
• (2) Deep-well microplate holders
• (2) 250ml bottles
• 50ml bottle
• 500ml waste bottle
• Computer running Fragment Analyzer controller and ProSize data analysis software
• Communication and power cables

Agilent 5300 Fragment Analyzer Configuration:

• Instrument Applications: NGS fragment analysis Plasmid DNA Large DNA fragments Genomic DNA Microsatellites/SSR Reverse Genetics TILLiNG (mutation detection)
• Analysis Method: fluorescence-based capillary electrophoresis using intercalating dye
• Capillary Array: compatible with 48 and 96 capillary arrays
• Minimum Sample Volume: 25 uL of liquid for injection; as little as 2.5 uL of actual sample required
• Sample Capacity: 3 sample trays, each holding 1x96-well PCR microplate
• Compatible Microplates: 96 well semi or non-skirted PCR plates for markers and samples, 96 deep-well plates for buffer and waste
• Number of Samples per Run: 48 or 96 (Depending on the installed Array)
• Variable Throughput: up to 288 samples (3 x 96-well plates) low-to-medium-throughput system
• High Sensitivity: sensitivities down to 5 pg/uL for a single fragment, cycle time, or PCR reagent
• Resolution and Sizing Accuracy: resolve small differences between fragments, as low as 3 bp for fragments under 300 bp size
• Power requirements: 100-240 VAC; 50/60 Hz
• Dimensions (H x W x D): 61 cm x 38 cm x 51 cm (24 x 14 x 20 in)
• Weight: 38.5 kg (85 lbs)

Customization/Options: N/A

Testing performed by our in-house service team:

The Agilent 5300 Fragment Analyzer passed all tests and was found to be fully functional:

• We checked the power supply to ensure it provides stable output.
• We tested communication with the software to verify seamless interaction and data exchange.
• We tested the initialization and homing processes to guarantee accurate and reliable setup.
• We checked the pump operation to ensure consistent and reliable fluid movement.
• We tested the plate movement to validate precise and smooth sample handling.
• We thoroughly tested the prime function of all fluids to ensure the system is clean and functions as designed.
• We tested the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
• We tested the high voltage system from minimum to maximum levels to verify consistent and safe operation.
• We performed an Optical Alignment on a Capillary Array, verifying the camera and illumination were operating fully.

Instrument Overview: 

• The Agilent 5300 Fragment Analyzer is a high-precision analytical instrument for DNA/RNA analysis.
• It uses parallel capillary electrophoresis to separate and analyze nucleic acids, enhancing workflow efficiency.
• The instrument can process multiple samples simultaneously, making it ideal for labs needing quick, reliable results.
• It provides detailed fragment size and concentration information with high sensitivity and resolution.
• Maintenance is simplified with an automated wash system and long-life capillaries.
• The Agilent 5300 minimizes user intervention, increasing throughput and reducing errors.

Instrument Application: 

• Nucleic acid analysis
• Fragment analysis
• Quality control in genomics research
• Microsatellite analysis
• SSR genotyping
• Analysis of PCR products

The Key Features:

• High-precision DNA/RNA analysis
• Advanced fragment analysis
• Low- to mid-throughput separation
• User-friendly interface
• Precise pump operation
• Controlled sample handling
• Seamless switching between applications

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER FRAGMENT ANALYZERS FROM US?

They want to ensure the power supply has been verified for stable output, preventing unpredictable voltage drops or surges that could prevent proper fragmentation of samples.

They want assurance that a full optical alignment of the capillary array has been performed, verifying that the camera and illumination system are operating properly for accurate detection and baseline stability.

They want to ensure the fluidic pump operates smoothly and consistently, avoiding pressure fluctuations that can lead to migration failures, sample cross-contamination, or incorrect fragment sizing.

They want to confirm that plate transport and positioning mechanisms have been validated for smooth, accurate movement, preventing needle crashes, missed wells, or bent sampling probes.

They want to confirm that electronic noise and EMI levels from the high-voltage supply remain within tolerance and will not distort the image signal quality.

They want to ensure that the electronics are up to date and compatible with the current software versions.

They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.  see questions section below  
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

SM Small Crate
225#

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper.  If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.  

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries. 

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.