Thermo Attune NxT Acoustic Focusing Cytometer (1 YEAR WARRANTY) with (4)Lasers/(14) Colors/(16)Detectors w/ CytKick MAX Autosampler

Internal part number 64067

For Sale Thermo Attune NxT Acoustic Focusing Cytometer (4)Lasers/(14) Colors/(16)Detectors w/ CytKick MAX Autosampler 

• Fully Functional
• Made in 2023
• Serviced & in Stock
• Expert sales consultation
• Includes remote installation and instrument training session
• Includes subscription to De Novo FCS Express 7 Analysis software
• 45 DAYS INTERNATIONAL WARRANTY

Optional Services

• Optional remote installation support and training are available for non-local customers 

Service Highlights

•  Calibration beads test results match expected values
•  Laser power output confirmed stable over time
•  Instrument stress testing performed

Configuration Highlights

•  Setup with (4)Lasers/(14) Colors/(16)Detectors - Max configuration
•  Includes a complementary subscription to De Novo FCS Express 7 Analysis software

Here is what you will receive:

• Thermo Attune NxT Flow Cytometer A29009 config (Comparable to A29004)
• CytKick Max Autosampler with Focus & Waste Bottles
• PC running Attune NxT Software ver 6.1.29 (w/ monitor, mouse, and keyboard)
• Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

Thermo Attune NxT Acoustic Focusing Cytometer Configuration:

Optics:(4) Lasers, (14) Colors, (16) Detectors:

• Forward Scatter Channel: 488/10 blue laser
• Side Scatter Channel: 488/10 blue laser  (Blue) 488/10 OD2 (SSC),
• 405nm violet laser: 450/40, 525/50, 610/20, 710/50, 660/20, 780/60
• 488nm blue laser: 530/30, 695/40
• 561nm yellow laser: 585/16, 620/15, 780/60
• 637nm red laser: 670/14, 720/30, 780/60
• Sample input rates: ~1 sample/minute
• Flow Rate: 12.5 to 1,000 μL/min
• Sample Analysis Volume: 20ul - 4mL
• Particle Size Range: 0.5 - 50um
• Data Acquisition Speed: up to 35,000 events/second with 34 parameters
• No-wash, no-lyse sample preparation workflow
• Cycle time: 20-40 min for tube

CytKick MAX Autosampler Configuration:

• Minimum Sample Volume: 50 μL (70 μL at 1000 μL/minute)
• Dead Volume: 30 μL (except 50 μL at 500–1000 μL/min)
• Minimum Sample Acquisition Volume: 20 μL
• Throughput: 22 min for 96-well plate (boost mode, using one rinse, one mix, and full analysis of a 20 µL sample)
• Carryover: <0.5% in standard mode using 2 wash cycles; <1% in Boost Mode
• Compatibility: 

  • 96 deep-well
  • 96-well standard depth
  • 384 deep-well
  • 384-well standard depth
  • 1.5 mL and 2 mL microcentrifuge tube rack (up to 24 per rack)
  • 96-well foil-covered
  • 384-well foil-covered customizable to accept additional plate types

• Cooling: passive cooling available for 96-well U-bottom plates and microcentrifuge tube racks

Customization/Options: 

• Attune NxT Extended Fluid System EFS1 for extended operation
• Other filter colors may be available (Please inquire for filter options in stock)

Testing performed by our in-house service team:

The Thermo Attune NxT Acoustic Focusing Cytometer passed all tests and was found to be fully functional:

• We verified the startup and initialization process.
• We connected the Thermo Attune NxT Acoustic Focusing Cytometer to the software.
• We ran the fluidics priming and startup sequences.
• We performed extensive cleaning of the fluidic system.
• We tested and confirmed a stable connection between the CytKick MAX Autosampler, the Attune NxT, and the computer.
• We assessed the operation of both the stage and needle motors.
• We evaluated the pumps to ensure optimal performance.
• We performed the laser alignment procedure.
• We conducted a baseline test using Attune Performance Beads.
• We checked overall instrument performance with Attune Performance Beads.

Instrument Overview:

• The Thermo Attune NxT Acoustic Focusing Cytometer (4 Lasers/14 Colors/16 Detectors) delivers acoustic focusing for superior sample alignment.
• The system supports up to four spatially separated lasers and modules enabling up to 14 fluorescent colors across 16 detection channels.
• Configured optics include lasers at 405 nm, 488 nm, 561 nm, and 637 nm, each paired with dedicated band-pass filters for optimal signal separation.
• The Thermo Attune NxT Acoustic Focusing Cytometer integrates an autosampler (CytKick MAX) for automated plate loading across multiple well-formats.
• The instrument’s benchtop size, robust fluidics, and versatile optical configuration make the Thermo Attune NxT Acoustic Focusing Cytometer suitable for demanding multi-parameter workflows.

Instrument Application:

• Cell sorting and analysis in immunology research
• Gene expression profiling in molecular biology
• Detection of Rare Cell Populations in Oncology Studies
• Multiplexed protein expression analysis in drug discovery
• Characterization of circulating tumor cells in cancer research
• Immunophenotyping of immune cell subsets in vaccine development

The key features:

• Four lasers for multi-color analysis
• 14 colors for comprehensive assays
• 16 detectors for high sensitivity
• Autosampler for increased throughput
• Accurate and precise sample handling
• Versatile for diverse research needs

 De Novo FCS Express Software Features

• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 Benefits of the Complimentary DeNovo FCS Express Software

• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
• Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Note: Subscription is for the Research Use Only (RUO) version. 

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER FLOW CYTOMETERS FROM US?

They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.

They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.

They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.

They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.

They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.

They want to ensure that no fiber optic cables are missing, misrouted, or defective, which can lead to diminished fluorophore excitation or signal dropout.

They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.

They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.

They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.

They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.

They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.

They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.  see questions section below  
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper.  If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.  

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries. 

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.