Agilent NovoCyte Penteon Flow Cytometer (5)Lasers/(30)Colors/(32)Detectors w/ NovoSampler Q & Fluidics Station II

Internal part number 62780

For Sale Agilent NovoCyte Penteon Flow Cytometer (5)Lasers/(30)Colors/(32)Detectors w/ NovoSampler Q & Fluidics Station II

• Fully Functional
• Serviced & in Stock
• Current Model
• Expert sales consultation
• Includes remote installation and instrument training session
• Includes subscription to De Novo FCS Express 7 Analysis software
• Warranty

Optional Services

• Optional remote installation support and training are available for non-local customers 

Service Highlights

•  Calibration beads test results match expected values
•  Laser power output confirmed stable over time
•  Instrument stress testing performed

Configuration Highlights

•  Includes 5 Laser, 96-well plate sampler
•  Includes a complementary subscription to De Novo FCS Express 7 Analysis software

Here is what you will receive:

• Agilent NovoCyte Penteon (U7V7B6Y6R4)
• Agilent NovoSampler Q
• Agilent NovoCyte Fluidics Station II
• Fluidics Station II bottles/caps, fluid harness, cables
• Desktop computer with NovoExpress software
• Complimentary De Novo FCS Express analysis software (1-Year Subscription) RUO version
• Communication and power cables

When you purchase a flow cytometer from Boston Industries, you also receive a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software, enhancing your research capabilities with these key benefits:

• User-Friendly Interface: Navigate complex data analysis easily using FCS Express's intuitive design.
• Advanced Analysis Tools: Utilize sophisticated tools for in-depth data exploration and interpretation.
• Comprehensive Reporting: Generate detailed, customizable reports that are publication-ready.
• High Compatibility: Supports multiple file formats, including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter files, Accuri C6 files, SPADE, Cytobank files, and TXT files.
• Enhanced Data Visualization: Create high-quality graphs and plots to visualize your data effectively
• With FCS Express 7, you will have the latest Research Use analysis software.

Agilent NovoCyte Penteon Flow Cytometer Configuration:

Optics:(5)Lasers/(30)Colors/(32)Detectors:

• Laser Color Allocation (Laser/Channel Count): UV/7, Violet/7, Blue/6, Yellow/6, and Red/4
• Forward Scatter Channel: 488 blue laser
• Side Scatter Channel: 488 blue laser
• 349nm UV Laser: 445/45 nm, 525/45 nm, 586/20 nm, 615/20 nm, 667/30 nm, 725/40 nm, 780/60 nm
• 405nm Violet Laser: 445/45 nm, 525/45 nm, 586/20 nm, 615/20 nm, 667/30 nm, 725/40 nm, 780/60 nm
• 488nm Blue Laser: 525/45 nm, 586/20 nm, 615/20 nm, 667/30 nm, 695/40 nm, 725/40 nm
• 561nm Yellow Laser: 586/20 nm, 615/20 nm, 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm
• 637nm Red Laser: 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm
• Laser: solid-state laser with onboard thermal-electric cooling and guaranteed thermal stability and beam quality
• Laser beam profile: 10 × 60 um elliptical beam
• Laser power: 349 nm 20 mW, 405 nm 100 mW, 488 nm 100 mW, 561 nm 100 mW, 637 nm 100 mW
• Fluorescence detection: silicon photomultiplier (SiPM) with high photon detection efficiency, individual photodetector for each channel
• FSC/SSC sensitivity: FSC: 0.4 um, SSC: 0.1 um
• Fluorescence threshold sensitivity: FITC ≤40 MESF, PE≤10 MESF, APC ≤10 MESF, Pacific Blue ≤30 MESF
• Fluorescence resolution <3% CV for CEN

Fluidics:

• Sample acquisition rate 100,000 events/second
• Sample delivery: positive-displacement syringe pump
• Volumetric absolute count precision <5%
• Sample flow rate: 5 to 120 μL/min, continuously adjustable
• Sheath flow rate: 6.5 mL/min
• Sample acquisition volume: 5 uL to 5 mL
• Manual sample format: 12×75 mm tube, 1.5 mL Eppendorf tube
• NovoSampler Q sample compatibility: 40-tube rack for 12×75 mm tube, 24-well, 48-well, 96-well (flat, U-, V-bottom), and 384-well microplates.
• Fluid level sensing: Active sensing using weight sensors with automated warnings when any fluid level is out of the specified range.
• Fluid container capacity: 3 L sheath, 3 L waste, 500 mL cleaning, 500 mL rinse
• Carryover: <0.1%

Customization/Options: N/A

Testing performed by our in-house service team:

The Agilent NovoCyte Penteon Flow Cytometer passed all tests and was found to be fully functional:

• We started up and initialized the NovoCyte.
• We ensured a connection to the software with each module.
• We executed fluidics priming, startup, and shutdown sequences.
• We performed extensive cleaning of the fluidics system.
• We ran several cleaning cycles, both manual and software-driven.
• We calibrated and tested the functionality of the Auto Sampler.
• We confirmed the functionality of the Fluidics cart sensors.
• We conducted a baseline test with multi-peak beads.
• The NovoCyte successfully passed the QC bead test.
• We perform PM before shipping.

Instrument Overview:

• Agilent NovoCyte Penteon Flow Cytometer with 5 lasers for multiparameter analysis.
• Configuration supports up to 30 fluorescence colors and 32 detectors.
• Integrated NovoSampler Q enables automated sampling from multi-well plates.
• Fluidics Station II ensures stable delivery and reliable sample handling.
• Agilent NovoCyte Penteon offers flexible panel design and high sensitivity.
• Built to support demanding research workflows and complex assays.

Instrument Application:

• Immunophenotyping diverse cell populations
• High-content multiparameter immune profiling
• Automated plate-based sample screening

The key features:

• 5 lasers for broad excitation options
• Up to 30 colors for complex panels
• 32 detectors for multiparameter analysis
• NovoSampler Q supports plate sampling
• Fluidics Station II ensures steady flow
• High sensitivity for rare cell detection

 De Novo FCS Express Software Features

• Data Import and Handling: Easily imports data from various flow cytometry instruments, supporting a wide range of file formats.
• Intuitive Interface: User-friendly interface with customizable dashboards for streamlined workflow management.
• Advanced Data Analysis: Provides comprehensive tools for complex data analysis, including statistics, gating, and kinetic analysis.
• Visualization Tools: Offers a variety of plotting options, such as histograms, scatter plots, and heat maps, for effective data visualization.
• Automated Reporting: Facilitates the creation of detailed reports with integrated data and graphics, automating repetitive tasks.
• Real-Time Data Updates: Supports real-time data analysis and visualization, enabling immediate insights and decision-making.
• Collaboration Features: Allows sharing of data and analysis results with team members through secure cloud-based platforms.
• Integration Capabilities: Seamlessly integrates with other laboratory software and databases for efficient data management and workflow integration.

 Benefits of the Complimentary DeNovo FCS Express Software

• Enhanced Data Management: Streamlines data import, handling, and analysis, improving overall data management efficiency.
• User-Friendly Design: Intuitive interface design ensures ease of use, reducing the learning curve and increasing productivity.
• Comprehensive Analysis Tools: Advanced analysis tools allow for in-depth examination of complex data sets, leading to more accurate results.
• Flexible Visualization Options: Wide range of plotting options helps in effectively visualizing data, facilitating better understanding and presentation.
• Time-Saving Automation: Automated reporting and analysis features save time and reduce manual effort, enhancing workflow efficiency.
• Immediate Insights: Real-time data updates enable quick insights and decision-making, crucial for time-sensitive experiments.
• Collaboration and Sharing: Secure cloud-based collaboration features promote data sharing and teamwork, enhancing research collaboration.
• Seamless Integration: Integration capabilities with other software and databases ensure smooth workflow and data management.

Note: Subscription is for the Research Use Only (RUO) version. 

WHY DO CUSTOMERS MAKING CRITICAL LAB PURCHASES CHOOSE TO ORDER FLOW CYTOMETERS FROM US?

They want to ensure the cytometer undergoes full fluidics system decontamination, flushing, and inspection to protect the valves and flow sensors from damage.

They want to ensure the cytometer has no residual sheath salt buildup in the flow cell, which could skew scatter data and, and if improperly cleaned, can damage the flow cell.

They want to confirm that all detectors, including forward scatter (FSC) and side scatter (SSC) detectors, can be aligned and calibrated for particle sizing and granularity resolution.

They want assurance that baseline performance has been verified using standardized particles, with data or log files available for review.

They want to confirm that electronic noise and EMI levels at the detector/amplifier stage remain within tolerance and will not distort signal fidelity.

They want to ensure that no fiber optic cables are missing, misrouted, or defective, which can lead to diminished fluorophore excitation or signal dropout.

They want to confirm that no power degradation or misalignment in laser paths is detected through precise mW-level measurement at the final optics output.

They want to ensure that optical filters and dichroic mirrors have been inspected for delamination, spectral shift, or contamination that could compromise signal specificity.

They want to ensure the sample flow rate is linear and stable across a full range of flow settings, verified with volumetric calibration and pressure profiling.

They want to ensure the system passes automated QC and performance tracking with reproducible CVs for bead standards across multiple channels.

They want to ensure the fluidics pump assembly does not exhibit pulsation, drift, or backpressure due to check valve wear or micro-leaks.

They want peace of mind, knowing their instrument is backed by a warranty from a company that can stand behind it.

Our Advantage

• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.  see questions section below  
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies. 
• Technical sales consultation available
• Remote installation and instrument operation training may be available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)

Our Guarantee

• Warranty
• You get after-sales technical support
• Customer references are available
• The instrument will be fully functional!
• Smooth process from the initial quote to order fulfillment

This will ship via freight. Please contact us with your zip code to obtain a shipping quote.

ME Medium Crate
460#

PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE

Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.

Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.

Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.

Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.

Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.

Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.

Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.

Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper.  If a customer ships on their account, they handle insurance.

Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.  

Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries. 

Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.