Used THERMO FISHER SCIENTIFIC CellInsight NXT

High Throughput Cell Based Screening Cell insight with Orbitor stacker

Optical bench: Thermo Scientific high efficiency epi-fluorecent optical train

Stage: Automated with plate insert.

Camera: High-resolution Photometrics X1 CCD, 14-bit camera, with large field of view and 4.54micron/pixel resolution.

Barcode Reader: Laser-scanning barcode reader.

Objectives: Olympus 10x (0.3NA) and 20x (0.45NA), Additional 2.0x and 4.0x

Light Source: Four color solid- state LED light engine for use with Hoescht, FITC, TRITC, and Cy5 fluorophores.

Software: HCS Studio 

CellInsight Instrument PC: Dell Precision T5600, 825W, Genuine Windows 7 Professional, 64-Bit OS, Four Core Xeon E5-2643, 3.3GHz Processor, 8GB, DDR3 RDIMM Memory

Dell 24" High Resolution Widescreen Monitor.

Highlights of the CellInsight NXT:

• Transmitted Light - designed to work with or without fluorescent labeling, the new CellInsight NXT allows users to explore more cell biology without the restriction of fluorescent dyes 
• Affordable - automated high content imaging for all budgets 
• Easy to Use - quickly build and optimize image-based assays with included iDev* software 
• Fast Performance - scan and analyze a 96-well plate in under 4 minutes 
• Quality - high quality imaging and virtually maintenance-free operation 
• Compact - fits on a bench and less than 2 ft. on all sides 

Capabilities of the CellInsight NXT:

• Make individual cell, subcellular and multicellular measurements 
• Measure area, size, shape and texture using intensity and location of probes 
• Make multiple measurements per object, over time if desired 
• Measure subpopulations and reject undesired objects automatically 

Easy to Use Applications:

• Comprehensively pre-validated and user configurable image analysis without scripting or programming 
• Exploratory assay development tools and specific biology optimized tools 
• Define biological thresholds for outputs based on control populations 
• Ability to create multi-parameter “gates” from individual output measurements