Product Details
| Model | BioFlo 320 |
| Brand | EPPENDORF |
| Category | Bioreactors |
| Posted | 23.02.2026 |
| Views | 688 |
Used - Good EPPENDORF BioFlo 320
USASeller:
trustregionalapproved
USA
The Company Boston Industries Inc. based in the USA and specialized on the recovery and sale of used laboratory and medical equipment.
Description
For Sale Eppendorf BioFlo 320 Dual Bioprocess Control System for Single-Use Vessels
Internal part number SM101332-2SU
- Fully Functional
- Serviced & in Stock
- Current Model
- Technical sales consultation available
- 60 DAYS INTERNATIONAL WARRANTY
- Includes remote instrument setup and operation training session
Bioreactors are configured to meet individual customer requirements (shown with BioBlu single-use vessels)
We will be able to configure this bioreactor for either microbial production or bioprocessing
Below is the configuration of the Bioflo 320 controller you will receive
Compatible vessel types and volumes
| Bioflo 320 controllers can be paired | Glass/Autoclavable vessels are 1.0L, 3.0L, 5.0L, or 10 Liters. |
| Bioflo 320 controllers can be paired | Eppendorg BioBlu single-use vessels are 1c, 1f, 3f, 3c, 5c, 5p,10f, 10c, 14c, or 50c |
Gas flow supply connections
| Gas flow control | TMFC (Thermal Mass Flow Controllers) |
| Gas mixing option | 4 gases (Air, N2, O2, CO2) |
| (Gas-in) Supply ports | (Air, O2 , N2 , CO2 ) Maximum 10 psig (0.69 barg) when paired with glass / autoclavable vessels Maximum 6 psig (0.44 barg) when paired with a BioBlu Single-use vessel |
| (Gas-out) Sparge connection | 3 TMFC; Flow Range: (0.002 – 1.0 SLPM) Also available as 0.04 – 20 SLMP |
| Optional: (Gas-out) Overlay connection | 1 TMFC; Flow Range: (0.05 – 5 SLPM), |
* Please note we also carry BioFlo 320 controllers with 0.04 to 20.0 SLPM Mass Flow Sensors
Bioflo 320 Controller digital connections/inputs
| Vessel drive | Accepts direct or magnetic drive connection port |
| Temperature sensor | Accepts PT100 temperature probes |
| Extra digital sensors (Two) | Accepts PH, DO, Redox, or CO2 sensors (maximum 2 sensors) |
| Heated blanket | Heating blankets can only be used with BioBlu vessels |
| Peltier exhaust condenser | Accepts different-sized Peltier exhaust condensers |
| Vessel Scale connector | Accepts weight range scales to measure vessel content |
| Level Sensors (Three) | Three available level inputs, one of which may be used for foam |
| Optional: Optical PH | An optional connector for optical PH may be added |
Bioflo 320 Controller peristaltic pumps
| Includes 4 installed peristaltic pumps |
|
| Pumps 1, 2, & 3 / Watson-Marlow 114DV head | with a variable speed: 5 – 25 rpm; Fixed Speed: 25 rpm (0 – 100% Duty Cycle) |
| Pump 4: Watson-Marlow 314D head | with a variable speed: 20 – 100 rpm; Fixed Speed: 100 rpm (0 – 100% Duty Cycle) |
| Optional add-on: up to two external pumps | May be paired with an optional Watson Marlow 120u peristaltic pump (fifth pump) |
Installed liquid connections and their operating temperature ranges
| External water source | Main water supply and return/drain (Chiller as option) |
| Vessel supply and return |
|
| Liquid-cooled exhaust condenser supply & return | 0.5 psig (0.035 barg) |
Controller Weight/Dimensions/Power Requirement
| Power requirements | 100-120/208-240 VAC |
| Dimensions (W x D x H) | 40.6 x 40.6 x 66.0 cm (16 in x 16 x 26 in) |
| Net weight: | 32 kg (70 lb) |
Here is what you will receive:
- Eppendorf Bioflo 320 Bioprocess Control Station (Qty:2)
- ELO Touchscreen Monitor w/ Arm Mount (Qty:1)
- Bioreactor Drive agitation motor (Qty:2)
- 4-gas regulator w/ valves (Qty:1)
- Heat Blanket (Qty:2)
- pH Sensor with cable (Qty:2)
- DO Sensor with cable (Qty:2)
- Temperature Sensor with cable (Qty:2)
This bioreactor is shown with an optional scale for BioBLU vessels
You can pair this Bioflo 320 controller with single-use Eppendorf BioBLU vessels for cell culture or microbial applications
This configuration includes the necessary components to use this controller with BioBLU vessels
* Please note the BioBlu vessel shown is not included as it is a single-use consumable.
| Eppendorf BioBlu c single use-vessels (for Cell Cultures) | BioBLU 0.3c (380 mL) | BioBLU 1c (1.8 L) | BioBLU 3c (5 L) | BioBLU 10c (13.3 L) | BioBLU 50c (5 L) |
| Working volume | 100 – 250 mL | 320 mL – 1.25 L | 1.25 – 3.75 L | 3.3 –10 L | 18 – 40 L |
| Number of impellers | 1 | 1 or 2 | 1 or 2 | 1 | 1 |
OR
| Eppendorf BioBlu f single use-vessels (for Microbial Cultures) | BioBLU 0.3f (380 mL) | BioBLU 1f (1.8 L) | BioBLU 3f (5 L) |
| Working volume | 65 – 250 mL | 250 mL – 1.25 L | 1.25 – 3.75 L |
| Number of impellers | 2 | 2 or 3 | 3 |
* Please note we also have this configuration with Autoclavable glass non-Jacketed vessels and Jacketed vessels for use with general water supply or external chiller. Please see all reactors in the “Bioreactors category”
Reactor Customization/Options might be available upon request. Please inquire with our sales team.
- Different Volume Vessels
- Choice of agitation impellers (pitched blade, marine, Rushton)
- We also have systems with sparge TMFC (Mass Flow Sensors) that are calibrated for 0.04 – 20 SLMP
- External chiller (heating/cooling)
- Additional ELO Touchscreen Monitor
- Cooling loops or cooling fingers for temperature control
- Redox or CO2 sensors
- Second 4-gas regulator kit
- Exhaust condenser (liquid or peltier)
- Jacketed vessels
- Vessel Scale
- * Source Scales
- Sparge and/or Overlay vessel attachment
- Vessel Baffle
- Level / Foam sensors
- Others accesories
Please note that, depending on the requested options, we may sometimes recommend a different model bioreactor that may better fit your application.
Testing performed by our in-house service team:
The BioFlo 320 bioprocess control system passed all tests and was found to be fully functional:
- We verify the power supply and complete the system initialization process.
- We assess the functionality of the touchscreen display, confirming responsive and accurate input.
- We test the agitation motor and motor control, confirming smooth and stable operation at multiple RPM ranges.
- We test Pumps 1 through 4 to ensure reliable and accurate delivery rates, including external pumps added to the system.
- We verify the pH sensor readings using a calibration solution, confirming accurate output.
- We verify the dissolved oxygen (DO) sensor readings using a calibration solution, ensuring sensor accuracy.
- We test the heating control system and verify temperature accuracy using a NIST-traceable calibrated probe.
- We test the gas output via the sparge line and verified gas control functionality.
- We test all add-on options and accessories.
- We perform additional testing as requested within reasonable parameters.
Instrument Overview:
- The Eppendorf BioFlo 320 Bioprocess Control System is a versatile and compact unit for various bioprocessing needs.
- Features a modular design with interchangeable control modules for flexibility and scalability.
- Integrates advanced process control for parameters like pH, DO, temperature, and agitation.
- Supports autoclavable and BioBLU single-use bioreactors for varied bioprocessing needs.
- Eppendorf BioFlo 320 offers a touchscreen interface and smart sensor integration for efficient operation.
- Designed for lab to pilot scale-up, the BioFlo 320 simplifies upstream bioprocessing workflows.
Instrument Application:
- Cell culture and fermentation
- Microbial growth optimization
- Scale-up and scale-down studies
- Biopharmaceutical production
- Process development and optimization
The Key Features:
- Designed for both microbial and cell culture applications
- Supports interchangeable autoclavable and BioBLU SU Bioreactors
- Advanced control of key process parameters
- Integrated peristaltic pumps for feeding
- Intuitive touchscreen interface for operation
- Real-time parameter monitoring
REASONS WHY THESE CUSTOMERS CHOOSE TO ORDER FROM US
- They want to ensure that the bioreactor controller has been thoroughly tested and responds to commands
- They want to ensure that the paired drive will be correctly matched to the required vessel size and type
- They want to ensure that the vessel’s drive RPMs are verified against the tachometer
- They want to ensure that the bioreactor controller can detect all attached sensors
- They want to ensure that all peristaltic pumps are tested up to their maximum RPMs
- They want to ensure that the internal heating coil and fluid pump are performing properly
- They want a reliable warranty
- They want to ensure that the included vessel is correctly matched to the bioreactor controller
- They want peace of mind knowing it will be safely and professionally packed for shipping
Our Advantage
- We have experience servicing and repairing these instruments.
- This instrument goes through all the described service steps before shipping. see the questions section below
- We offer detailed photos and videos of the instruments you buy.
- We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
- We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
- Technical sales consultation available
- Remote installation and instrument operation training may be available.
- On-site installation may be available in the Boston Area (New England area)
- US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
Our Guarantee
- Warranty
- An extended warranty may be available
- You get after-sales technical support
- Customer references are available
- The instrument will be fully functional!
- Smooth process from the initial quote to order fulfillment
This will ship via freight. Please contact us with your zip code to obtain a shipping quote.
PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.
Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.
Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.
Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.
Q: Do you ship Internationally?
A: Yes! We have shipped to multiple continents and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 60% or more and have plenty of funds left over for an additional instrument or two.
Didn’t find what you were looking for? Place your own ad on the Bimedis platform
Place your advert on Bimedis and reach buyers worldwide.